West Java, Indonesia, stands out as a hub of banana diversity, serving as the national center for banana cultivation and seed production. This province boasts an extensive array of banana genotypes, including banana muli, king, kepok, horn, and more, with over half of the region dedicated to banana production. Despite this rich diversity, many banana genotypes remain poorly characterized, leaving a vast potential genetic resource untapped. Among these diverse banana varieties, banana muli stands out for its myriad benefits. Notably, it serves as an excellent energy source due to its high-calorie content, rapidly absorbed by the body. Additionally, banana muli varieties are rich in essential minerals, vitamins, iron, and folic acid. Furthermore, they possess significant levels of resistant starch, classified as prebiotics, which remain undigested and promote the growth of beneficial gut bacteria. This study aims to shed light on the genetic diversity of banana muli in West Java, providing valuable insights into its potential applications and benefits. By characterizing this banana genotype, we contribute to the broader understanding of this vital crop's genetic resources and its potential for various uses, including as functional food.