Prostatitis and benign prostate hyperplasia (BPH) are common prostate-related conditions that affect many men. Oxidative stress has been implicated in the development and progression of prostate disorders, including prostatitis, and is associated with the generation of reactive oxygen species (ROS). Antioxidants play a crucial role in mitigating oxidative damage and have been studied for their potential therapeutic effects on prostate health. This study investigates the antioxidant potential of Moringa oleifera, a plant known for its rich phytochemical content, in an animal model of BPH induced by testosterone. The enzymatic activities of three key antioxidants—Malondialdehyde (MDA), Superoxide Dismutase (SOD), and Glutathione (GSH)—were measured spectrophotometrically to assess the plant's ameliorative properties. The results demonstrated a significant increase in antioxidant activity with Moringa oleifera treatment, as evidenced by elevated levels of MDA, SOD, and GSH. A statistically significant difference in mean GSH levels was observed on days 10, 20, and 25 of treatment (p<0.05), suggesting the plant’s potential to mitigate oxidative stress associated with BPH. These findings support the use of Moringa oleifera as a potential therapeutic agent for prostate-related disorders and highlight the importance of antioxidants in reducing oxidative damage linked to prostate diseases