Guiera senegalensis is a medicinal plant used for the treatment of various illnesses. The study was aimed to investigate the phytochemical constituents and antibacterial activity of Guiera senegalensis root extracts. Extraction was conducted with the aid of soxhlet Extractor. Phytochemical screening was carried out using conducted by conventional method. The antibacterial activity of the extracts was investigated using agar well diffusion method while broth dilution method was used to determine minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) of the extracts. The phytochemical screening of the extracts revealed the presence of saponin, flavonoid, alkaloid, tannin, steroid, terpenoid and glycoside. The antibacterial activity of the extract depends on its concentration and types of isolates. Highest zone of inhibition is demonstrated by S. aureus (14.25 mm), E. coli recorded 13.33 mm while Salmonella was least sensitive with 12.66 mm. The ethanol extract was more active against the isolate with average zone of inhibition of 15.58 mm followed acetone has 13.16 mm while ethyl acetate extract has an average zone of inhibition of 11.83 mm. it is concluded that the extracts of G. senegalensis possessed antibacterial agents