The study's goal was to learn more about the positive effects of antioxidants combination on male infertility. Twenty-four men with semen low parameters (subfertility) and another 10 men normally used as control were used in this study. Males were given formulation of an antioxidant containing vitamin E 400 mg, vitamin C 500 mg, l-carnitine 1,000 mg, zinc 20 mg, selenium 0.20 mg, daily (n = 24) or a placebo (n = 10). sperm parameters and sperm DNA fragmentation index improved significantly after three months of treatment in the internal pilot study. The current results showed that antioxidants have therapeutics effects on patients with subfertility, they showed enhancement in sperm concentration, total sperm number, motility, normal forms as well as DNA fragmentation. In conclusion, antioxidants can increase the fertility and treat men with subfertility